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KMID : 0613820070170111523
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Journal of Life Science
2007 Volume.17 No. 11 p.1523 ~ p.1532
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Ginseng Saponin-Re and Coix lachrymajobi var. mayuen Regulate Obesity Related Genes Expressions, TNF-alpha, Leptin, Lipoprotein Lipase and Resistin in 3T3-L1 Adipocytes
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Kim Sung-Ok
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Abstract
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In order to determine if the mRNA and protein expression levels of 3T3-L1 adipocytes are influenced by oriental medicines, adipocytes were treated with 100 μg/ml of G-Re and aqueous extract of a Coix lachrymajobi var. mayuen (AEC) every other day for 12 days, respectively. The tumor necrosis factor alpha (TNF-α). mRNA and protein expressions were suppressed markedly in treated mature adipocytes. Those of lipoprotein lipase (LPL) levels were found to increase gradually in preadipocytes differentiating into mature adipocytes. Those were higher than that of the untreated mature adipocytes. The treated adipocytes showed reduction of leptin expression levels, while in untreated mature adipocytes cell, those of levels were significantly higher after the conversion of preadipocytes into mature adipocytes. The resistin levels in the treated adipocytes were significantly decreased comparing to that of the untreated mature adipocytes. In conclusion, the expression levels of LPL, TNF-α, leptin and resistin mRNA and proteins are shown to be regulated by G-Re and AEC, making them potential candidates for controlling fat mass related obesity.
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KEYWORD
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3T3-L1 adipocyte, TNF-α, lipoprotein lipase, leptin, resistin
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